rabbit anti-cbd-28k  (Cell Signaling Technology Inc)

Journal: Antioxidants

Article Title: Therapeutic Administration of Oxcarbazepine Saves Cerebellar Purkinje Cells from Ischemia and Reperfusion Injury Induced by Cardiac Arrest through Attenuation of Oxidative Stress

doi: 10.3390/antiox11122450

Figure Lengend Snippet: ( A , C ) CBD-28k immunohistochemistry ( A ) and FJB histofluorescence ( C ) in the cerebellar vermis of the sham + vehicle ( A a, C a). The CA + vehicle ( A b–d, C b–d), sham + OXC ( A e, C e) and CA + OXC ( A f–h, C f–h) groups at 12 h and one and two days after CA/RoSC. In the CA + vehicle group, the numbers of CBD-28k-Purkinje cells are apparently reduced (arrows) in the Purkinje cell layer (PCL) two days after CA/RoSC, and FJB-cells are apparently increased (arrows) in the PCL at 12 h after CA/PCR. In the CA + OXC group, CBD-28k- and the number of FJB-Purkinje cells are markedly saved and reduced, respectively, two days after CA/RoSC. GCL, granular cell layer; MCL, molecular cell layer. Scale bar = 50 µm. ( B , D ). Numbers of CBD-28k-Purkinje cells ( B ) and FJB-Purkinje cells ( D ). The bars determine the means ± SEM ( n = 7, respectively; * p < 0.05 versus sham, † vehicle group; # p < 0.05 versus CA, † vehicle group).

Article Snippet: In short, the cerebellar sections were blocked with 20% normal goat serum (in 0.05 M PBS) and immunoreacted with primary rabbit anti-CBD-28k (diluted 1:1100; Cell Signaling Technology, Danvers, MA, USA), mouse anti-4HNE (diluted 1:450; Alexis Biochemicals, San Diego, CA, USA), sheep anti-SOD1 (1:1500; Calbiochem, Darmstadt, Germany) and sheep anti-SOD2 (1:1500; Calbiochem) at 4 °C for eight hours.

Techniques: Immunohistochemistry